Enzyme Histochemical Expression of Lipoprotein Lipase in the Liver and Adrenal Glands in Clinically Healthy Rabbits
نویسندگان
چکیده
The liver enzyme lipoprotein lipase (LPL) facilitates the release of cholesterol from lipoproteins and plays a key role in the synthesis of steroid hormones in the adrenal glands. For the current study, 6 male and 6 female clinically healthy New Zealand White rabbits, aged 3.5 months were used. Enzyme histochemical reaction (per Gomori) was performed on fresh cryostat sections, and through the Tween method, a strong positive expression of LPL in the sinusoid capillaries of the liver lobules, the lumen, and the smooth muscle cells of the walls of interlobular arteries and veins was shown. A moderate LPL expression in zona glomerulosa and weak expression in zona fasciculata were observed in the adrenal glands, whereas no LPL expression was found in both the cortical zona reticularis and the adrenal medulla.
منابع مشابه
Distribution of Tissue Lipoprotein Lipase Activity in Feline Bulbourethral Gland Structures
The aim of the present study was a demonstration of LPL activity and localization in feline bulbourethral glands. The glands of seven sexually mature, clinically healthy European shorthair male cats, aged 12-18 months, weighing 2.8-4 kg, obtained from a licensed animal breeder were investigated in the present study. Cryostat cross sections of 5-7 μm were used for enzyme histochemical detection ...
متن کاملFunctional molecular mass of rat hepatic lipase in liver, adrenal gland and ovary is different.
Lipoprotein lipase (LPL) is functionally active only as a dimer. It is also generally assumed that the highly homologous hepatic lipase functions as a dimer, but no clear evidence has been presented. A hepatic lipase-like activity, also indicated as L-type lipase, is present in adrenal and ovary tissues. This enzyme is thought to originate from the liver and to be identical to hepatic lipase. W...
متن کاملEffects of methylphenidate on the mice Adrenal glands and lymphoid organs: Results of histochemical, histometrical and histopathological investigations
BACKGROUND: Considering widely administration of methylphenidate and also its immunosuppressive effects on different organs, importance of related microscopic studies is obvious. OBJECTIVES: Determining histological effects of methylphenidate on adrenal glands and lymphatic organs in mice. METHODS: A total number of 30 adult male Balb/C mice were provided, weighed and divided into one control a...
متن کاملHistochemical study of river buffalo’s uterine endometrium in follicular and luteal phases
This study was conducted to evaluate histochemical alterations of river buffalo’s uterine wall acid andalkaline phosphatases (ALP and ACP), lipase and carbohydrate ratio during follicular and luteal phases.Forty apparently healthy and non pregnant river buffalos were considered for removal of the uterus aftersacrifice in slaughterhouse. All dissected uteri were divided into two luteal (by obser...
متن کاملSynthesis of hepatic lipase in liver and extrahepatic tissues.
Immunoprecipitations of hepatic lipase from pulse-labeled rat liver have demonstrated that hepatic lipase is synthesized in two distinct molecular weight forms, HL-I (Mr = 51,000) and HL-II (Mr = 53,000). Both forms are immunologically related to purified hepatic lipase, but not to lipoprotein lipase. HL-I and HL-II are also kinetically related and represent different stages of intracellular pr...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2011